One of the biggest challenges today for infrastructure administrators is how to provide a secure, yet productive and supportable, environment. As many organizations begin planning and deploying Microsoft Application Virtualization 4.5 (App-V), an understanding of the security capabilities and best practices for administrative and operational tasks is important for any infrastructure administrator. Security of the App-V system relies on proper setup of the software and the environment in which it operates. This document covers, describes, and provides guidance for configuring the various components of App-V from a security perspective. Administrators should carefully consider their exposure and attack surface before deciding to deploy a system without the security recommendations outlined in this document.